年報2004

I. Outline of the Noda Institute for Scientific Research

II. Achievement of Research Activities in 2004

Reproduced Papers
T. Takahashi, O. Hatamoto, Y. Koyama and K. Abe
Efficient gene disruption in the koji-mold Aspergillus sojae using a novel variation of the positive-negative method
 Reproduced from Mol Gen Genomics, 272: 344-352 (2004) by copyright permission of the Springer-Verlag
A. Ventosa, M. C. Gutiérrez, M. Kamekura, I. S. Zvyagintseva and A. Oren
Taxonomic study of Halorubrum distributum and proposal of Halorubrum terrestre sp. nov.
 Reproduced from Int. J. Syst. Evol. Microbiol., 54: 389-392 (2004) by copyright permission of the International Union of Microbiological Society
T. Mizuki, M. Kamekura, S. DasSarma, T. Fukushima, R. Usami, Y. Yoshida and K. Horikoshi
Ureases of extreme halophiles of the genus Haloarcula with a unique structure of gene cluster
 Reproduced from Biosci. Biotechnol. Biochem., 68: 397-406 (2004) by copyright permission of the Japan Society for Bioscience, Biotechnology, and Agrochemistry
Y. Boucher, M. Kamekura and W. F. Doolittle
Origins and evolution of isoprenoid lipid biosynthesis in aechaea
 Reproduced from Mol. Microbiol., 52: 515-527 (2004) by copyright permission of the Blackwell Publishing Ltd
T. Mizuki, M. Kamekura, M. Ishibashi, R. Usami, Y. Yoshida, M. Tokunaga and K. Horikoshi
Nucleoside diphosphate kinase of halobacteria―Amino acid sequence and salt-response pattern
 Reproduced from J. Japn. Soc. Extremophiles, 3: 18-27 (2004) by copyright permission of the Japan Society for Extremophiles
H. Tokunaga, K. Mitsuo, M. Kamekura and M. Tokunaga
Major outer membrane proteins in moderately halophilic eubacteria of genera Chromohalobacter and Halomonas
 Reproduced from J. Basic Microbiol., 44: 232-240 (2004) by copyright permission of the John Wiley & Sons, Inc.
Y. Boucher, C. J. Douady, A. K. Sharma, M. Kamekura and W. F. Doolittle
Intragenomic heterogeneity and intergenomic recombination among haloarchaeal ribosomal RNA genes
 Reproduced from J. Bacteriol., 186: 3980-3990 (2004) by copyright permission of the American Society for Microbioligy
M. Akahira-Azuma, M. Szczepanik, R. F. Tsuji, R. A. Campos, A. Itakura, N. Mobini, J. McNiff, I. Kawikova, B. Lu, C. Gerard, J. S. Pober and P. W. Askenase
Early delayed-type hypersensitivity eosinophil infiltrates depend on T helper 2 cytokines and interferon-gamma via CXCR3 chemokines
 Reproduced from Immunology, 111: 306-317 (2004) by copyright permission of the Blackwell Publishing Ltd
T. Koga, P. Kwan, L. Zubik, C. Ameho, D. Smith and M. Meydani
Vitamin E supplementation suppresses macrophage accumulation and endothelial cell expression of adhesion molecules in the aorta of hypercholesterolemic rabbits
 Reproduced from Atherosclerosis, 176: 265-272 (2004) by copyright permission of the Elsevier Science Ltd
A. Oda, C. Sakuta, S. Masuda, T. Mizoguchi, H. Kamada and S. Satoh
Possible Involvement of Leaf Gibberellins in the Clock-Controlled Expression of XSP30, a Gene Encoding a Xylem Sap Lectin, in Cucumber Roots
 Reproduced from Plant Physiology, 133: 1779-1790 (2004) by copyright permission of the Springer-Verlag
Papers in Japanese
K. Matsushima, T. Takahashi, Y. Hanya and Y. Koyama
Molecular Biological study for Non-producibility of Aflatoxin by Koji mold Aspergillus sojae
 Reproduced from J. Japan Soysauce Research Institute, 30: 5-11 (2004) by copyright permission of the Japan Soysauce Research Institute
S. Hara, R. F. Tsuji, O. Hatamoto and T. Masuda
A Simple Method for Enrichment of Uninucleate conidia of Aspergillus oryzae
 Reproduced from J. Japan Soysauce Research Institute, 29: 229-234 (2003) by copyright permission of the Japan Soysauce Research Institute
Japanese Patents Laid-Open
Titles of Oral and Poster Presentations

III. Report on the Research Assisted by the NISR Research Grant in 2003

Research Grant
Harushi NAKAJIMA (The University of Tokyo)
Cell surface engineering in Aspergillus oryzae
Hirofumi SHOUN (The University of Tokyo)
Basic studies on fungal unique P450 for industrial applications
Masao TOKUNAGA (Kagoshima University)
Development of highly soluble enzymes adopting the structural characteristics of halophilic enzymes
Masaaki WACHI (Tokyo Institute of Technology)
Analysis of the role of E. coli RNA-binding protein Hfq in the regulation mechanism of gene expression
Yoh WADA (Osaka University)
Modification vacuolar function and physiology of Saccharomyces cerevisiae by expressing mammalian V-ATPases
Young Investigator Research Grant
Shin HARUTA (The University of Tokyo)
Microbial ecological studies on traditional food fermentation
Takane KATAYAMA (Kyoto University)
Functional analysis of the proteins involved in the aromatic amino acids metabolism in bacteria
Jun-ichi MARUYAMA (The University of Tokyo)
Functional characterization of AoHex1 involved in Woronin body formation in Aspergillus oryzae
Hiroyasu ONAKA (Toyama Prefectural University)
Characterization of the biosynthetic gene cluster of goadsporin which promote secondary metabolism and sporulation in actinomycetes.
Yumiko TAKATSUKA (Tohoku University)
Molecular dissection of the Selenomonas ruminantium cell envelope and lysine decarboxylase involved in the biosynthesis of a polyamine covalently linked to the cell wall peptidoglycan layer
Shinji TAKENAKA (Kobe University)
Characterization of aerobic denitrification mechanism in Klebsiella pneumoniae strain F-5-2 that can utilize ammonium and nitrate ions simultaneously and application of nitrifying and denitrifying processes to wastewater treatment

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