Annual Report 2018

I.Achievement of Research Activities in 2018


I. Nishimura, Y. Shinohara, T. Oguma, Y. Koyama
Survival strategy of the salt-tolerant lactic acid bacterium, Tetragenococcus halophilus, to counteract koji mold, Aspergillus oryzae, in soy sauce brewing.
Biosci, Biotechnol, Biochem., 82: 1437-1443 (2018)

K. Ishikawa, E. Kunitake, T. Kawase, M. Atsumi, Y. Noguchi, S. Ishikawa, M. Ogawa, Y. Koyama, M. Kimura, K. Kanamaru, M. Kato, T. Kobayashi
Comparison of the paralogous transcription factors AraR and XlnR in Aspergillus oryzae
Current Genetics, 64: 1245–1260 (2018)

T. Takahashi, M. Ogawa, A. Sato, Y. Koyama
Translocated duplication of a targeted chromosomal segment enhances gene expression at the duplicated site and results in phenotypic changes in Aspergillus oryzae
Fungal Biol. Biotechnol. 5: 17 (2018)

Y. Eguchi, H. Kodama, H. Kudo, M. Hosaka, H. Shindo, M. Ogawa, Y. Koyama, A. Yanase, G. Arakawa, M.Tokuoka
A unique Zn(II)2-Cys6-type protein, KpeA, is involved in secondary metabolism and conidiation in Aspergillus oryzae.
Fungal Genetics and Biology, 127: 35-44 (2019)

Y. Shinohara, I. Nishimura, Y. Koyama
Identification of a gene cluster for biosynthesis of the sesquiterpene antibiotic, heptelidic acid, in Aspergillus oryzae
Biosci, Biotechnol, Biochem., DOI:10.1080/09168451.2018.1549934 (2019)

M. Zhuang, Z.-M. Zhang, L. Jin, B.-T. Wang, Y. Koyama, F.-J. Jin
The bHLH transcription factor DevR significantly affects polysaccharide metabolism in Aspergillus oryzae
Appl. Environ. Microbiol., DOI: 10.1128/AEM.00089-19 (2019)

II.Grant project

NISR has started a new project, which supports a research fund in the field of applied biology for the purpose of developing the fermentation and brewing industry.

1) 2018 NISR Research Grant

Hideki AOYAGI(University of Tsukuba)
Development of effective method for isolating and culturing of non-culture microbe using specialized cellulose sheet

Shumpei ASAMIZU(The University of Tokyo)
Biosynthesis and action mechanism of sideromycin class antibiotic from Streptomyces sp

Yoshiharu INOUE(Kyoto University)
Role of TOR signaling pathway in freeze-thaw stress response in yeast

Hiromi MAEKAWA(Kyushu University)
Application of sexual mechanism of methylotrophic yeast in the artificial mating type switching system

Hiroyuki TACHIKAWA(The University of Tokyo)
Roles of membrane contact sites during prospore membrane formation of budding yeast

Youhei YAMAGATA(Tokyo University of Agriculture and Technology)
Exploring transcriptional factors that controlled transcription of aspartic endopeptidase and their DNA binding quences

2) 2018 NISR Young Investigator Research Grant

Takashi FUJISHIRO(Saitama University)
Development of an Escherichia coli-based whole-cell catalyst expressing metalloenzymes responsible for anaerobic CO2 fixation

Kentaro FURUKAWA(Niigata University)
Omics analysis of degradation targets by mitochondrial autophagy

Yuki HONDA(Nara Women’s University)
Light-driven NAD(P)H regeneration system for microbial bio-production

Daisuke IMAMURA(Hosei University)
Development of a bioreactor system using Bacillus subtilis spores

Emi KUNITAKE(Mie University)
G-protein/cAMP signaling pathway-mediated repression of genes encoding polysaccharide-degrading enzymes in filamentous fungi

Ken-Ichi OINUMA(Osaka City University)
Identification and analysis of a hydrazide-degrading enzyme of mycobacteria

3) 2017 NISR Research Grant

Yoichi HONDA(Kyoto University)
Mechanism for mRNA maturation control in basidiomycetes

Shingo IZAWA(Kyoto Institute of Technology)
Study on preferential translation under pronounced translation repression caused by brewing-related stress in Saccharomyces cerevisiae

Hiroshi KADOKURA(Tohoku University)
Identification of PDI family members required for the production of useful proteins

Takuji OKA(Sojo University)
Fundamental research on the biosynthesis of fungal-type galactomannan (FTGM) as a new drug target

Masahiro OKADA(The University of Tokyo)
Screening of secondary metabolites by genome shuffling

Sachiko TSUKAMOTO(Kumamoto University)
Production of new biologically active compounds by a combined culture of fungi and investigation of their activation mechanism

4) 2017 NISR Young Investigator Research Grant

Naoya KATAOKA(Yamaguchi University)
Metabolic engineering of coryneform bacteria for the enhanced supply of acetyl-CoA and its application to the fermentative production of useful compounds

Akimasa MIYANAGA(Tokyo Institute of Technology)
Functional analysis of post-PKS enzymes in hitachimycin biosynthesis

Kenji OKANO(Osaka University)
Construction of a platform strain for anaerobic bioprocessing and its application to biofuel production

Motoyuki SHIMIZU(Meijo University)
Proteomics-based screening and functional characterization of novel enzymes involved in the degradation of wood components

Yota TSUGE(Kanazawa University)
Production of 1,5-diaminopentane and succinate in a single Corynebacterium glutamicum strain via a bidirectional metabolic switch controlled by the oxygen level

Back to Annual Reports